In 2016; Uday Singh et al 2015). Cow ghee

In developingcountries, people more rely on herbal based medicine for primary health care.

Inrecent years, herbal medicine and their active components are gainingpopularity to encounter various metabolic diseases such as obesity, heart diseaseand diabetes etc., (Butt et al 2009).In generally believe that herbal medicines are natural, no toxic or side effectsand immensely contribute to health benefits (Adeyemi et al 2014).  Alliumsativum, commonly known as garlic is one of the ancient medicinalherbs and most heavily studied herbs over the past several decades and the reportis mostly good.

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  Garlic holds a uniqueactive substance which contributes to wide range of pharmacological effect includinganti hyperlipidemic effect (Majewski,2014). There is evidence that hypolipidemic influences of garlic showssignificantly reduce serum and hepatic total cholesterol, triglyceride, andlow-density lipoprotein levels and increase serum high-density lipoproteinlevels (Niaz et al, 2016; Uday Singh etal 2015).Cow ghee orclarified butter from milk solid could be consumed as food and as medicine.

Itcontains high energy, essential fatty acids and fat soluble vitamins (Ganguli and Jain 1973). Cow ghee is a traditionalmedicine in Ayurveda and which are practiced for thousands of years, apart fromits role in the daily use of culinary purposes and traditional religious rituals.Cow’s ghee holds numerous medicinal properties and many of its combinationswith herbs are described in traditional texts. Karandikar et al 2016; Shukla and Tripathi 2006; Murthy Srikantha et al1998).

Despite this fact, ghee contains higher amount saturated fatty acidswhich causes cardiovascular risks were reported. Evidence on the health effectsof ghee and garlic are individually reported by Indian system of medicine,which discusses the possible benefits. However, there is no scientific data hasbeen made in regard to the hypolidemic of garlic and its combination with ghee.These spurt interest to develop a dietary intervention of Garlic infused ghee toexplore their synergistic therapeutic effects in rodent model. In the pastdecades, hyperlipidemia and associated complications have reached epidemic proportions.For the study of this pathophysiology, several rodent models have beendeveloped. High-fat diet model is the one to induced obesity in rodent by highcalorie intervention method.

The word ‘high fat diet’ is first introduced by Masek& Fabry in 1959. These animal models enhance the increase glycemicindex and insulin resistances on both muscle and liver. Based on thisobservation, high-fat diets can be used to valuable tool to rodent model forthe metabolic syndrome (Buettner et al 2006).Therefore, present study was undertaken to ascertain whether the combination ofboth garlic and ghee produces synergistic effect in hyperlipidemic condition.