This given 8 ounces of a syrupy glucose solution

test measures how well the body handles a large amount of glucose. The glucose
assay is used for the quantitation of glucose in human serum, plasma, urine, or
cerebrospinal fluid(CFS). The health care provider draws the patient’s blood
before and two hours after the patient drinks a large, premeasured beverage
containing glucose. Then, the doctor can compare the before-and-after glucose
levels contained in the person’s plasma to see how well the body processed the
sugar. These levels are measured in mg/dL.

preparation for OGTT, patients should be advised to eat a normal carbohydrate
diet (>150g daily) for at least 3 days prior to the test and undertake
normal physical activity. Patients must fast for 10-14 hours prior to this test
but may drink small volumes of plain water. Smoking and physical exercise
should not be allowed in the morning prior to, and during, the test. The test
should be performed in the morning, and patients should remain at rest during
the test. A blood sample to measure the patient’s baseline
glucose level, and this test is called the fasting glucose test. After the
fasting glucose test is completed, the patient is given 8 ounces of a syrupy
glucose solution that contains 75 grams of sugar. The patient should consume
the glucose solution within 5min, and 1-2 mL blood should be taken in a
fluoride oxalate tube. 1-2ml of blood should be collected from the patient again
after 2h.

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the diagnosis of gestational diabetes, the procedure is slightly different.
Instead of consuming a 75g worth of sugar solution, a 50g glucose solution is
given to the pregnant woman instead. Besides using the same 2h OGTT test
already described, an additional blood draw is added at the 1h mark for the 2h
OGTT procedure. The second step is generally only conducted if the first step
has a positive result. The positive result in step one is usually a blood
glucose level of either greater than 92mg/dL for fasting glucose, or greater
than 180mg/dL 1h after consumption of glucose solution, or greater than
153mg/dL 2h after ingestion of glucose solution, or a combination of all
three.   Step two is a is a three-hour version of the
OGTT, where the pregnant patient must consume a syrupy glucose solution that
contains 100 grams of sugar. They’ll draw the patient’s blood when she is
fasting and at the one, two, and three-hour marks after she has drunk the
glucose solution.

 The glucose assay is used for the quantitation
of glucose in human serum, plasma, urine or cerebrospinal fluid(CFS). Blood
glucose determinations are the most frequently performed clinical chemistry
laboratory procedures, commonly used as an aid in the diagnosis and treatment
of diabetes. Elevated glucose levels (hyperglycemia) may also occur with
pancreatic neoplasm, hyperthyroidism, and adrenal cortical hyperfunction as
well as other disorders. Patient samples need to be diluted accordingly before
loading into the machine for testing.